Cyclists are expected to gather on Friday evening on Dunkeld Corner to ride for Burry Stander, who was killed in an accident.
Stander collided with a taxi in KwaZulu-Natal on Thursday while cycling on Shelly Beach in Port Shepstone on his mountain bike.
Critical Mass' Melvin Neil says this is also to remember all cyclists who died on the country's roads.
"The purpose is to show that there are thousands of people who want to ride their bicycles in Johannesburg and to show that there is no infrastructure and that there's a lot of ignorance on the part of cyclists and the part of motorists."
Cyclists will also be gathering in Cape Town to take part in an evening ride throughout the Mother City in Stander's memory.
The one hour tour will start at the Green Point turning circle at 6pm.
Cycling group Moonlight Mass is using Twitter to spread the word about the ride.
Its co-founder Elad Kirshenbaum says, "Everyone will split into groups and ride around the city for an hour to show their support as well as ride for safety."